Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment. Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.This F-12K (Kaighn's) Medium is manufactured as follows:With: L-glutamine, Phenol RedWithout: HEPESThe complete formulation is available.Using Ham's F-12KHam's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH. Order Info Shipping Condition: Room Temperature Greener Choice ClaimsEnvironmental benefits include:Recycled ContentRenewable MaterialsSource ReductionRecyclable

Key	Value
Cell Type	Human Hepatocytes
Classification	Animal Origin-free
Concentration	1 X
Form	Liquid
Product Type	Ham's F-12K (Kaighn's) Medium
Sterility	Sterile-filtered
Sterilization Method	Sterile-filtered
With Additives	Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate
Without Additives	No HEPES
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Quantity	10 x 500 mL
Shipping Condition	Room Temperature
Content And Storage	Storage conditions: 2-8°C. Protect from lightShipping conditions: Ambient