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1M Sulphuric Acid

1M Sulphuric Acid

Code: HI-ML187-100ML

Enquire about stock levels

Average lead time: 03-09-2026

Lead time: approx. 3 to 4 weeks

100ML

€6.09 ex VAT

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Description

1M Sulphuric acid can be used as a Stop solution in ELISA where 3,3',5,5'-tetramethylbenzidine (TMB). acts as a substrate. Sulfuric acid inactivate the enzyme. Once the enzyme gets inactivated, the reaction will stop. Sulfuric acid changes the pH of surrounding medium so drastically that the enzyme present in the solution cannot act on its substrate hence the reaction stops. 1M Sulphuric Acid serves as a standard stop solution for colorimetric assays using TMB or other acid sensitive substrates. It facilitates enzymatic reaction termination to allow for stable absorbance readings in molecular diagnostic research screening and quantitative analysis.

Sulphuric acid plays a major role in ELISA experiments as a Stop solution where 3,3’,5,5’, tetramethylbenzidine (TMB) acts as a substrate. Stop solution should fulfil two major requirements: 1) to terminate the reaction by effectively inhibiting the enzymatic activity of HRP, 2) to stabilize the oxidized products of the chromogenic or fluorogenic substrates. In the case of TMB, the stop solution, Sulphuric acid, not only inhibits color development but also converts the blue oxidation product of TMB to a yellow derivative which has an absorbance maxima at 450mn.

Specifications

  • Brand: HiMedia
  • Product Code: HI-ML187-100ML
  • Pack Size: 100ML
  • Lead Time: Approx. 3 to 4 weeks

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