Thermo Scientific™ Pierce™ Premium Grade TCEP-HCl, 10 g

The highest quality formulation of Pierce TCEP-HCl, a thiol-free, odourless and stable reducing agent for cleaving protein and peptide disulfide bonds. Premium Grade adds enhanced testing, batch-specific documentation, lot retention and change control notification, making it ideal for applications where product integrity and risk minimisation are critical.

Premium Grade Features

• High Quality: Identity and purity confirmed by several tests, including quantitative NMR
• Product Integrity: Enhanced testing and characterisation compared with standard grade
• Lot Retention: Ample samples of past lots retained to support future process testing
• Change Management: Change Control Notification (CCN) service
• Consistent Manufacture: Batch-specific manufacturing documentation review

Advantages over Other Reducing Agents

• Odourless: Unlike DTT or BME, reductions can be carried out conveniently on the bench
• Stable in Air: No special precautions needed to prevent oxidation during handling, use or storage
• Efficient: 5 to 50 mM typically reduces disulfide bonds within a few minutes at room temperature
• Selective: Completely reduces even the most stable water-soluble alkyl disulfides over a wide pH range
• Compatible: Removal is usually unnecessary before downstream steps such as His-tag protein purification and maleimide conjugation

Product Details

• Chemical Name: Tris(2-carboxyethyl)phosphine hydrochloride
• Product Type: Disulfide reducing reagent
• Grade: Premium
• Form: Crystalline solid
• Product Line: Pierce™
• Pack Size: 10 g

Storage and Shipping

• Storage: Room temperature, in a sealed container to prevent oxidation
• Shipping: Ambient